Redefining Reference Standards

HDx™ Reference Standards
Identify and Control Variability Every Day

DNA Extraction & Quantitation Performance Monitoring

Pre-analytical processing typically involves multiple steps, including sample fixation, DNA isolation, and DNA quantitation. At each of these stages, even minor differences in e.g. operator to operator sample handling, instrumentation and methodology can introduce significant variation, calling into question the quality of DNA and the reliability of downstream assay results.

  • The use of formalin fixative stems from its ability to cross-link proteins, however it has also been reported to induce methylol modifications and acid-mediated hydrolysis which can confound genotyping analysis
  • DNA extraction is usually performed using commercially available kits. Variation between manufacturers and even lot to lot from the same manufacturer is common
  • DNA quantitation is not performed by all laboratories but can be useful to ensure that DNA isolates meet minimum concentration for use in an assay. Measurement systems can be confounded by low sample yield and sample fragmentation.

In response to this need, Horizon Diagnostics has developed a range of HDx™ FFPE Reference Standards to enable effective end-to-end process validation. Thereference standards are highly consistent and reliable, yielding near identical quantities of DNA, enabling users to compare extraction kits and control for batch to batch variation, as seen in the figure below.

H+E staining & DNA Extraction demonstrates the homogeneity and consistency of our FFPE technology

For each FFPE block we analyze DNA extraction from multiple sections and perform an Aperio® digital scan to verify cell numbers per section. The figure shows data from DNA extracted from 10 sections, each from 8 independent FFPE blocks, together with the corresponding H+E stains.

If you’re having trouble finding what you’re looking for or require more information on this topic, please Contact Us.

Paragraph explaining benefits of Biocair

×

Login Error


There was an error with your login - please try again.

Forgotten your password? Click here

OK ×

Recover your password

Please enter your email address and we'll send you a reset link

×

Thank you.


We've sent you an email to reset your password.

OK ×

Add Voucher


Sorry, Unable to add voucher

OK ×

Account Error


There was an error updating your details.

OK ×

Thank you.


Your changes have been saved.

OK ×

Thank you


Thank you for registering to the Horizon DX website. We hope you enjoy and benefit from our online resources, and please feel free to contact us if you have any queries or feedback through the website itself or at info@horizondx.com.


×

Thank you.


Items have been saved to your basket.

OK ×