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View FISH HDx Reference Standards for Fluorescence In-Situ Hybridization
Fluorescence-based In-situ Hybridization (FISH) is used to visualize DNA or localized RNA in cells, tissues and tumors. Providing a reliable means for studying the genetic composition of cells in mitosis as well as interphase, with high detection sensitivity and capacity for multiplicity, FISH is also frequently used to demonstrate the presence of cancer gene rearrangements characteristic of certain hematologic malignancies.
Two main components are required in the preparation of a FISH assay: the DNA probe and the target DNA to which the probe will be hybridized. To date, a wide range of commercially available probes have been developed for a number of common targets, however their reliable use requires stringent validation and routine monitoring of assay performance. Consistent and well-validated reference materials are therefore essential to ensure accuracy.
Horizon Diagnostics has worked closely with the key European and US pathology proficiency schemes, FISH laboratories and biotech companies to develop FISH HDx Reference Standard slides. The reference materials are provided in cell culture spread or formalin-fixed, paraffin embedded (FFPE) sections as appropriate for the tumor type being evaluated.
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